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KMID : 1059519920360050753
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Journal of the Korean Chemical Society
1992 Volume.36 No. 5 p.753 ~ p.759
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The Development of Arginine-Selective Membrane Electrode Using Tissue Slices of the Rose of Sharon
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Kim Ui-Rak
Nam Ki-Jae
Choi Sung-Moon
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Abstract
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It is shown that receptacle tissue of the rose of sharon can serve as an effective biocatalyst by the construction of a potentiometric membrane electrode with response and selectivity for arginine. Electrodes using this tissue in conjunction with NH3 gas sensing probes yield good response to arginine in 10-1M to 10-3M range and retain activity for a least 1 weeks. The arginine-selective membrane electrode using tissue slices of the rose of sharon have good selectivity in presence of other amino acids. The optimum conditions are pH 8.0, 25℃ and 20 μm thickness of slices in 0.1 M phosphate buffer solution and Km values is 6.4 × 10-6(M).
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